pmcherry rab4a 7 addgene Search Results


pmcherry rab4  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
Pmcherry Rab4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmcherry rab4 - by Bioz Stars, 2026-07
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c1 rab11 addgene  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
C1 Rab11 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmcherry+rab4a+7+addgene/pm31054872-270-81-82?v=Addgene+inc
Average 93 stars, based on 1 article reviews
c1 rab11 addgene - by Bioz Stars, 2026-07
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2018 n a pmrfp c3 rab5 addgene  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
2018 N A Pmrfp C3 Rab5 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmcherry+rab4a+7+addgene/pm31054872-270-63-74?v=Addgene+inc
Average 93 stars, based on 1 article reviews
2018 n a pmrfp c3 rab5 addgene - by Bioz Stars, 2026-07
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beta1 mcherry f giancotti addgene 16042 rab9  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
Beta1 Mcherry F Giancotti Addgene 16042 Rab9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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beta1 mcherry f giancotti addgene 16042 rab9 - by Bioz Stars, 2026-07
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b3 integrin mcherry j jones  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
B3 Integrin Mcherry J Jones, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry rab2a j simpson ucd gpi  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
Mcherry Rab2a J Simpson Ucd Gpi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dsred rab9 wt r pagano  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
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mcherry calnexin m davidson  (Addgene inc)
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WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), <t>mCherry-Rab4</t> (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).
Mcherry Calnexin M Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), mCherry-Rab4 (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).

Journal: bioRxiv

Article Title: WDFY2 restrains matrix metalloprotease secretion and cell invasion by retention of VAMP3 in endosomal tubules

doi: 10.1101/299610

Figure Lengend Snippet: WDFY2 localization in the endocytic pathway. SIM image showing WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (grey) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEAl-labelled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 μm, scale bar of insets 1 μm (n = 14 cells). SIM images showing the localization of GFP-WDFY2 in relation to mCherry-Rab5 (n = 12 cells), mCherry-Rab4 (n = 13 cells), Anti-Rab7 (n = 14 cells) or mCherry-Rab11 (n = 14 cells). Scale bar: 1 μm, scale bar of insets: 0.5 μm. Protein-Lipid overlay assay using purified full length WDFY2. WDFY2 binds with high selectivity to PtdIns(3)P. Deconvolved widefield image showing GFP-WDFY2 localization to endosomes. GFP-WDFY2-R315A, a mutation in the binding site for PtdIns(3)P, abolishes the localization to endosomes and the protein is cytosolic. Scale bar: 10 μm (n=10 cells).

Article Snippet: The following plasmids were obtained from Addgene: pmCherry-Rab4 (55125) and pmCherry-WASH1-C-18 (55162) were a gift from Michael Davidson, pEGFP-VAMP3 (42310) was a gift from Thierry Galli . pmCherry-Cortactin (27676) and Coronin1B-mCherry (27694) were a gift from Christien Merrifield . pX458 (48138) was a gift from Feng Zhang .

Techniques: Marker, Protein-lipid Overlay Assay (PLOA), Purification, Mutagenesis, Binding Assay